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Modern Optical Spectroscopy: With Exercises and Examples from Biophysics and Biochemistry
Modern Optical Spectroscopy: With Exercises and Examples from Biophysics and Biochemistry
This textbook offers clear explanations of the theory of optical spectroscopic phenomena and shows how these ideas are used in modern molecular and cellular biophysics and biochemistry. The topics covered include electronic and vibrational absorption, fluorescence, resonance energy transfer, exciton interactions, circular dichroism, coherence and dephasing, ultrafast pump-probe and photon-echo spectroscopy, single-molecule and fluorescence-correlation spectroscopy, Raman scattering, and multiphoton absorption. The explanations are based on time-dependent quantum mechanics and are sufficiently thorough and detailed to be useful for both students and researchers. With the clear, thoroughly illustrated explanations that begin from first principles, the text will also be accessible to readers with little prior training in quantum mechanics. Extra details and highlights are featured in special boxes throughout the text. A helpful exercises section has been included to this new student edition. -
Applications of Infrared, Raman, and Resonance Raman Spectroscopy in Biochemistry
Applications of Infrared, Raman, and Resonance Raman Spectroscopy in Biochemistry -
Spectroscopy In Biochemistry
Spectroscopy In Biochemistry -
Time-Resolved Laser Spectroscopy in Biochemistry Iii, 20-22 January, 1992, Los Angeles, California (Proceedings of S P I E)
Time-Resolved Laser Spectroscopy in Biochemistry Iii, 20-22 January, 1992, Los Angeles, California (Proceedings of S P I E) -
Raman and Ir Spectroscopy in Biology and Biochemistry (Ellis Horwood Series in Analytical Chemistry)
Raman and Ir Spectroscopy in Biology and Biochemistry (Ellis Horwood Series in Analytical Chemistry)
Following an introduction to the theory of vibrational spectroscopy which describes the variety of methods used, this volume discusses the information obtainable from the spectra of proteins. It demonstrates how both classical and resonance Raman methods are used to ascertain protein structure, the properties of the active sites of enzymes and the nature of protein action. Of the many other types of compounds included, the nucleic acids are of particular interest, as Raman spectra can provide details not only of their structures, but also of their modes of interaction with metals, drugs, proteins and viruses. -
Time-Resolved Fluorescence Spectroscopy in Biochemistry and Biology (Nato a S I Series Series a, Life Sciences)
Time-Resolved Fluorescence Spectroscopy in Biochemistry and Biology (Nato a S I Series Series a, Life Sciences) -
Decomposition of plant tissue submerged in an extremely acidic mining lake sediment: phenolic CuO-oxidation products and solid-state ^1^3C NMR spectroscopy ... article from: Soil Biology and Biochemistry]
Decomposition of plant tissue submerged in an extremely acidic mining lake sediment: phenolic CuO-oxidation products and solid-state ^1^3C NMR spectroscopy ... article from: Soil Biology and Biochemistry]
This digital document is a journal article from Soil Biology and Biochemistry, published by Elsevier in 2004. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
In extremely acidic mining sediments of the Lusatian mining district, the alkalinisation process relies on organic C, which can serve as electron donor for microbially induced sulfate reduction. Plant material of the pioneer plant Juncus bulbosus is an important organic matter source in lake sediments. Therefore, decomposition of the plant tissue was assessed during the exposure of litterbags for 30 months in the 0-5 cm layer of waterlogged mining sediments, which have a pH between 2.5 and 3. The ash free dry weight (AFDW) and elemental content of the plant tissue were recorded several times during the exposure. Changes in chemical structure were analyzed by solid-state ^1^3C cross polarization magic angle spinning nuclear magnetic resonance (CPMAS NMR) spectroscopy and the lignin component characterized by wet-chemical CuO oxidation. The AFDW accounted for about 34% of initial biomass after field exposure for 30 months. Mass loss of biomass occurred in two phases with decomposition rates varying between 30 and 430 mg AFDW d^-^1. The mass loss increased considerably after 5-7 months when litterbags were invaded by fresh J. bulbosus plants. With respect to higher mass loss, ^1^3C CPMAS NMR spectroscopy, showed slight changes of the bulk chemical composition after 11 months, indicating that microorganisms present in the sediments or in the rhizosphere degrade plant material as a whole, rather than selectively. During the second phase from about 11 months until the end of the exposure period, contribution of O-alkyl C most probably assignable to easily degradable polysaccharides decreased. In contrast, the contribution of alkyl, aromatic and carboxyl C increased. CuO oxidation showed that the lignin component of J. bulbosus is degraded oxidatively during field exposure. Our results indicate that the exposed plant material is decomposed in the sediment due to changes in sediment conditions that followed plant invasion of the litterbags. It is suggested that the rhizosphere of J. bulbosus by its influence on the redox potential, pH and the microbial component plays a crucial role in organic matter degradation in acidic mining sediments. -
Quantifying soil organic carbon fractions by infrared-spectroscopy [An article from: Soil Biology and Biochemistry]
Quantifying soil organic carbon fractions by infrared-spectroscopy [An article from: Soil Biology and Biochemistry]
This digital document is a journal article from Soil Biology and Biochemistry, published by Elsevier in 2007. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
Methods to quantify organic carbon (OC) in soil fractions of different stabilities often involve time-consuming physical and chemical treatments. The aim of the present study was to test a more rapid alternative, which is based on the spectroscopic analysis of bulk soils in the mid-infrared region (4000-400cm^-^1), combined with partial least-squares regression (PLS). One hundred eleven soil samples from arable and grassland sites across Switzerland were separated into fractions of dissolved OC, particulate organic matter (POM), sand and stable aggregates, silt and clay particles, and oxidation resistant OC. Measured contents of OC in each fraction were then correlated by PLS with infrared spectra to obtain prediction models. For every prediction model, 100 soil spectra were used in the PLS calibration and the residual 11 spectra for validation of the models. Correlation coefficients (r) between measured and PLS-predicted values ranged between 0.89 and 0.97 for OC in different fractions. By combining different fractions to one labile, one stabilized and one resistant fraction, predictions could even be improved (r=0.98, standard error of prediction=16%). Based on these statistical parameters, we conclude that mid-infrared spectroscopy in combination with PLS is an appropriate and very fast tool to quantify OC contents in different soil fractions. -
Applications of Infrared Spectroscopy in Biochemistry, Biology and Medicine
Applications of Infrared Spectroscopy in Biochemistry, Biology and Medicine -
Time-Resolved Laser Spectroscopy in Biochemistry IV
Time-Resolved Laser Spectroscopy in Biochemistry IV
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