by Robert Beynon
Post-genomic analysis to establish gene function is a major challenge in biology research. Such analysis relies heavily on mass spectrometry, a technique unfamiliar to most biologists. This book describes mass spectrometry for those with little experience and also provides practical guidance on how to obtain protein samples, analyze them using mass spectrometry, and then describes the application of bioinformatics to determine sequences. A comprehensive range of applications are then covered, including: * recognition of known proteins * identification and characterization of unknown proteins * analysis of chemical and enzymatic modifications By providing both the background theory and the basic protocols, Mass Spectrometry takes the reader directly to the laboratory bench. In addition, the book also provides analytical chemists and others more familiar with mass spectrometry with an understanding of protein science and how best to apply these chemical techniques in their analysis.
Post-genomic analysis to establish gene function is a major challenge in biology research. Such analysis relies heavily on mass spectrometry, a technique unfamiliar to most biologists. This book describes mass spectrometry for those with little experience and also provides practical guidance on how to obtain protein samples, analyze them using mass spectrometry, and then describes the application of bioinformatics to determine sequences. A comprehensive range of applications are then covered, including: * recognition of known proteins * identification and characterization of unknown proteins * analysis of chemical and enzymatic modifications By providing both the background theory and the basic protocols, Mass Spectrometry takes the reader directly to the laboratory bench. In addition, the book also provides analytical chemists and others more familiar with mass spectrometry with an understanding of protein science and how best to apply these chemical techniques in their analysis.